Serine biosynthesis is a metabolic vulnerability in FLT3-ITD-driven acute myeloid leukaemia [iFLT3-ITD-Depletion_In VITRO]

We performed 3'-RNA-Sequencing on RNA isolated from murine MLL-AF9/iFLT3-ITD cells where the inducible iFLT3-ITD transgene had been depleted for 24 or 48 hours compared to non-depleted conditions in vitro to perform differential gene expression analysis. Cells were cultured in the presence of 1 µg/mL doxycycline to maintain iFLT3-ITD transgene expression in vitro. Cells were then washed twice with PBS and cultured in the absence of doxycycline to deplete iFLT3-ITD transgene expression for 24 or 48 hours (or maintained in 1 µg/mL doxycycline as a control). Total RNA was isolated from cells and 3'-RNA libraries were prepared for next-generation sequencing.