Single-Cell RNA-Seq of in vitro Expanded Cells from Cranial Neural Crest Reveals a Rare Odontogenic Subpopulation

Neural crest is highly heterogeneous. However, the cellular landscape of in vitro cultured CNC cells (CNCCs) for odontogenesis remains unclear. Here, via large-scale single-cell RNA sequencing (~ 59000 cells), we report the distinct cell trajectories of in vitro cultured mouse CNCCs from primary cells to passage 5.