The pattern, distribution and analyses of gene function of induced mutations in J. curcas using nGBS and ddGBS

Mutagenesis in combination with Genotyping by Sequencing (GBS) are powerful tools for introducing variation, studying genefunction and identifying causal mutations underlying phenotypes of interest in crop plant genomes. About 400 million paired-end reads were obtained from 82 ethylmethane sulfonate (EMS) induced mutants and 14 wild typeaccessions of J. curcas for the discovery of Single Nucleotide Polymorphisms (SNPs) and Insertion/Deletions (InDels) by two differentapproaches (nGBS and ddGBS) on an lllumina HiSeq 2000 sequencer.