NanoString nCounter NF1-mutant melanoma gene expression
收藏NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE200904
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Purpose: Digital spatial profiling was used to elucidate the interplay between the tumor cells and the surrounding microenvironment FFPE tissues. Methods: Two tissue microarrays (TMA) were generated from each FFPE block using the ISNET Galileo CK4500 computer-driven tissue microarray. Images of H&E-stained cores were demarcated by a pathologist and were utilized alongside NanoString immunofluorescent staining for morphology markers S100, CD45, CD3, and DAPI to draw region of interest. Multiplex gene expression analysis covers 73 endogenous genes from 24 different immune cell types and 48 gene-derived signatures measuring biological variables crucial to the tumor-immune interaction.
创建时间：
2022-04-16
