Ig_UHPLC_MS_submission raw data

Antibody glycosylation plays a crucial role in the humoral immune response by regulating effector functions and influencing binding affinity to immune cell receptors. While antibody glycosylation has been studied extensively for its potential to predict disease status, previous studies have focused mainly on the immunoglobulin G (IgG) isotype due to analytical challenges associated with other isotypes. Thus, developing a sensitive and accurate analytical platform is necessary to characterize antibody glycosylation across multiple isotypes. Our platform targets 98 glycopeptides across IgG, IgA, and IgM isotypes, as well as eight surrogate peptides representing total IgG, four IgG classes, two IgA classes, and IgM. The developed method is sensitive and accurate for Ig glycosylation profiling. It can be adopted for clinical investigations, particularly for detailed humoral immune profiling.