Potency of GNF7686 and prototypic cytochrome <i>b</i> inhibitors in <i>L</i>. <i>donovani</i>, <i>T</i>. <i>brucei</i>, <i>P</i>. <i>falciparum</i> and <i>S</i>. <i>cerevisiae</i> proliferation assays.

GNF7686 and cytochrome b inhibitors were tested for cytotoxicity in various parasites using the CellTiter-Glo Luminescent Cell Viability assay reagent system (see Table 1), SYBR Green Fluorescent dye (P. falciparum only), or MIC visual growth inhibition screen (S. cerevisiae only). Luminescence or fluorescence was monitored using the EnVision Multilabel Plate reader and EC50 values (with respective standard errors) were determined based on three (n = 3; L. donovani and T. b. brucei) or two (n = 2; P. falciparum and S. cerevisiae) biological repeats with duplicate technical repeats. Abbreviations include: BSF (bloodstream form), DHODH (malarial dihydroorotate dehydrogenase), yDHODH-D10attB (fumarate-utilizing S. cerevisiae DHODH), YPD (yeast extract peptone with dextrose), and YPG (yeast extract peptone with glycerol). Potency of GNF7686 and prototypic cytochrome b inhibitors in L. donovani, T. brucei, P. falciparum and S. cerevisiae proliferation assays.