Kinetics of osmotic stress regulate a cell fate switch of cell survival-Step200 -ID-101320-Jurkat cells exposed to hypertonicity

To understand the molecular changes of signaling and markers of cell state when cells are exposed to hypertonic stress, we measured time courses for the below markers: comb step200 combined comb step200 FSC_H comb step200 FSC_W comb step200 FSC_A comb step200 SSC_H comb step200 SSC_W comb step200 SSC_A combabb step200 CS_aCaspase8_PE combabb step200 BD_aCaspase3_A647 combaba step200 BD_pS6_A647 combaba step200 SC_NFAT5_A594 combabc step200 BD_aCaspase3_PE combabc step200 BD_cPARP_A647 combabd step200 BD_pH2AX_A555 combabd step200 BD_Ki67_A700 combabe step200 BD_pNfkb_A647 combabf step200 CS_pp38_A594 combabf step200 SC_pASK_A680
Conclusion:
Conclusions are described in: https://www.biorxiv.org/content/10.1101/2020.07.10.197871v1
Notes:
timepoints 0,2,5,10,15,20,30,60,120,180,240 300 min after addition of 300 mosmol/l NaCl are barcoded by 12 barcodes using Pacific-Blue (dilution: high, mid, low) and Pacific-Orange ((dilution: high, midhigh, midlow, low) following established protocols for fluorescent cell barcoding for flow cytometry: timepoints are barcoded as follows: PO high + PB high=0, PO midhigh + PB high=2, PO midlow + PB high=5, PO low + PB high=10, PO high + PB mid=15, PO midhigh + PB mid=20, PO midlow + PB mid=30, PO low + PB mid=60, PO high + PB low=120, PO midhigh + PB low=180, PO midlow + PB low=240, PO low + PB low=300