Comprehensive Analysis of Transcriptome in Dorsal Root Ganglia Following Chronic Constriction Injury.

To investigate mechanisms underlying neuropathic pain, we established sciatic nerve chronic constriction injury (CCI) neuropathic pain model in rats, and performed RNA-seq on ipsilateral bulk L4-L6 dorsal root ganglia from CCI rats and sham rats 11 days after surgery. By comprehensive analysis, we identified several key mRNAs and miRNAs, especially those associated with inflammatory immune response, may serve as promising targets, facilitating further investigation and eventually developing better therapeutic strategies for NP.为探究神经性疼痛的潜在机制，我们在大鼠中构建了坐骨神经慢性压迫损伤（sciatic nerve chronic constriction injury, CCI）神经性疼痛模型，并对术后11天的CCI模型大鼠与假手术大鼠的同侧大块L4-L6背根神经节开展了RNA测序（RNA-seq）。通过综合分析，我们鉴定出多个关键的信使RNA（mRNA）与微小RNA（miRNA），尤其是与炎症免疫反应相关的分子，或可作为极具潜力的治疗靶点，助力后续研究并最终开发出更优质的神经性疼痛治疗策略。