Development of acquired resistance to sotorasib in NCI-H358 cell line in vitro

NCI-H358 is a KRAS G12C-mutant non-small lung cancer cell line known to be sensitive to KRAS G12C-inhibitor sotorasib. Here, we aimed to develop sotorasib resistance in H358 and interrogate the resulting sotorasib-resistant cell line (H358-R). The H358-R cell line was created by continuous culturing of the H358 cells in sotorasib (250 nM) until cells grew readily under sotorasib pressure (approximately 2 months). Exome and transcriptome of the resulting sotorasib-resistant cell line (H358-R) were profiled through Illumina sequencing with and without sotorasib pressure, as well as compared with those of the parental sensitive cell line (H358).