Effect of mono-specific human anti-Ov-CPI-2 antibodies on L3 molting and viability in the presence of normal human neutrophils.

aO. volvulus L3 were cultured in complete medium alone or in complete medium containing negative control antibodies purified from the same serum donor using a λgt11 lysate column or mono-specific antibodies to rOv-CPI-2 in the presence of 2×105 normal human neutrophils per well for 6 days. Larval molting and viability were determined as described under the “Materials & Methods” section. Three experiments were performed, where each consisted of a total of 10 wells per treatment group with 5 larvae per well. The results are the mean ± SEM and the range of the three experiments.*Significance between groups was determined by unpaired t test with Welch's correction with p = 0.007 between medium control and the anti-Ov-CPI-2 group and p = 0.028 between the negative antibodies and the anti-Ov-CPI-2 group.**There is no significant difference between the three groups.