YC-1 inhibited Aβ25–35-induced μ-calpain, p25, and phosphorylated tau protein expression.

Effects of Aβ25–35 (10 µM) on the time-course changes in the expression of (A) μ-calpain, (C) p25, and (E) phosphorylated tau (p-tau) in differentiated PC12 cells. Differentiated PC12 cells were treated with Aβ25–35 (10 µM) for 24 h. All data shown represent the mean ±SD (n = 5). *P25–35 (10 µM) in differentiated PC12 cells. Differentiated PC12 cells were incubated with Aβ25–35 (10 µM) and YC-1 (10 µM) was added to the medium 30 min before the addition of Aβ25–35. All data are means ± SD of five independent observations with different cell passages and on different days. Depicted are a typical display of protein production (upper panel) and the statistical analysis of the changes of protein (lower panel). The typical data (β-actin) obtain from the same nitrocellulose membrane in Figures 3D and 3F. *P#P25–35-treated cells.