Integrative Multi-Omic characterization of LYN deficient HS-5 stroma cells

LYN kinase is a tyrosine kinase, that regulates cellular homeostasis in a context specific manner. Our group could show, that its expression in the leukemic microenvironment of chronic lymphocytic leukemia contributes to disease progression (Nguyen PH et al.; Cancer Cell; 2016). To analyze the effect of LYN kinase on the leukemia supportive phenotype of the bone marrow stromal cell line HS-5, we generated single cell clones of LYN deficient cells. These cells were analyzed in a Multi-Omic approach, comprising four "Omic" layers [microarray-based transcriptome, label-free quantitative proteome and secretome, and SILAC-labelled phosphoproteome (phospho-tyrosine (pYome))] and analyzed integratively.

Raw data of the single "Omic" layers are deposited in different repositories and linked below.
The results of the data analysis for every "Omic" layer can be found in the spreadsheets below.