Surface proteins identified in ≥50% clinical isolates by “shaving” Streptococcus pneumoniae cells followed by LC/MS/MS analysis.

aProtein categories were established according to LocateP subcellular predictions: lipoproteins were those predicted as lipid-anchored proteins; cell wall proteins, as those having an LPXTG motif; secretory proteins, as those possessing an SP1-type signal peptide; membrane proteins with one transmembrane domain (TMD), as those possessing either a C- or an N-terminally anchored transmembrane region; membrane proteins with more than one transmembrane domain, those predicted as multi-transmembrane proteins; “surface proteins” means the sum of the five previous categories; and cytoplasmic proteins, those without any exporting or sorting signal, and predicted as intracellular proteins.