Gene expression analysis of mouse pancreatic organoids

Producing endocrine cells is the foundation of next-generation treatments for organ dysfunction alternative to transplantation of pancreatic islets. In this study, we generated in vitro pancreatic organoid from Ngn3-GFP mice to investigate whether Ngn3 positive cells emerge from the pancreatic duct organoid. Pancreatic ducts were collected from the tissue and were subjected to organoid culture using matrigel which form 3D structure. 11 days after pancreatic duct culture, Ngn3 positive cells were observed in some of organoids. We have selected both Ngn3 positive and negative wells and extracted cDNA from each well (N=3) and RNA-Seq was performed using these samples. We found that organoids with abundant Ngn3 expression had potential to differentiate into endocrine cells.