Microsatellites of Primula vulgaris in translocated golf course populations

Background and AimsSpecies forced back into intensively used agricultural landscapes face severe disturbance and random destruction, making alternative restoration measures necessary. In Western Europe, Primula vulgaris, a perennial heterostylous herb, is nowadays restricted to fragmented habitats in arable landscapes. Translocations were conducted at five locations on a golf course area, using outcrossed juveniles originating from remnant populations. This study aimed to: (i) evaluate the golf course’s contribution to species conservation; (ii) assess source population suitability; and (iii) determine the translocation network's impact on genetic diversity and gene flow of neighbouring populations.
MethodsWe conducted a demographic (census size, demographic structure, morph ratio) and genetic study (genetic diversity and structure, connectivity network, barrier and parentage analyses), using 13 microsatellite loci across adult and juvenile generations of translocated, adjacent, and rem..., Translocations were conducted at five locations on a golf course area, using outcrossed juveniles originating from remnant populations.
Only transplants obtained from legitimate outcrosses (between morphs) were used. Translocations were conducted (1) in G1 in 2007 with 1-year old transplants (N =180); (2) in G2 and G3 in 2008, with plantlets taken from G1 –that had rejuvenated and increased in size– (N = 100); (3) in 2009 in G4 with plantlets from G1 (N = 140), and for enlarging G2 (an additional N = 25); and (4) in 2013 in G5, with plantlets taken from various previously translocated Golf populations (N = 30)., , # Microsatellites of Primula vulgaris in translocated golf course populations

[https://doi.org/10.5061/dryad.2bvq83c15](https://doi.org/10.5061/dryad.2bvq83c15)

## Description of the data and file structure

**DNA extraction and microsatellite analysis**

Genomic DNA was extracted from approximately 20 mg of dried leaf tissue using the E.Z.N.A. SP plant DNA Mini kit (Omega Bio-Tek, Norcross, GA, USA). Thirteen microsatellite markers (PRIV4, PRIV7, PRIVB13, PRIVB15, PRIVB17, PRIVB19, PRIV21, PRIV27, PRIVB29, PRIVB34, PRIVB35, PRIVB36, and PRIVB39) previously developed (Van Geert *et al.*, 2006; Triest *et al.,* 2015) were amplified by multiplexed polymerase chain reactions (PCR) following Triest *et al.* (2024). Primers were fluorescence-labelled with four different dye-labels (6FAM, VIC, NED, and PET). DNA concentration was 20–50µg/ml. A primer mix was made by mixing 0.2 µM of each primer together. Multiplex PCRs consisted of 6.25 µl master mix (Qiagen Multiplex PCR kit), 1.25 µl prim...,