Distribution of pheromone precursors, pheromone receptors and homeodomain genes in F. velutipes monokaryotic siblings.

Distribution of pheromone receptors and pheromones was analyzed in monokaryotic F1 progeny from dikaryon KACC43778 (DK). Presence of genes is indicated by ‘+’ and absence by ‘-‘. FvSTE3.s1 and FvSTE3.s2 show the distribution of their respective, non digested PCR products. Their subtypes, genes that were identified in sequence analysis of FvSTE3.s1 and FvSTE3.s2 are RFLPs1-a (a), RFLPs1-b (b), RFLPs2-c (c) and RFLPs2-d (d). Subtypes were detected by restriction length polymorphism analysis of PCR products. The table shows specific linkage of pheromone receptor FvSTE3.1 and pheromone FvPP1 with the matB3 locus. Copies of FvSTE3.s1 and FvSTE3.s2 recombine in pairs but independent from the mating-type loci; FvSTE3.s1-a is coupled to FvSTE3.s2-d and FvSTE3.s1-b is coupled to FvSTE3.s2-c. Homeodomain genes FvHD2-1 and FvHD2-2 co-segregate specifically with the matA3 locus. Segregation of FvSTE3.2, FvSTE3.s3, FvSTE3.s4, FvSTE3.s5, FvHD1-1, FvPP2 and FvPP3 could not be determined. Copies of those genes were detected in all mating-types.