Presence of FLT3-ITD and mutated NPM1 in ALDHbright and ALDHlow compartments in CD34-positive AML.

Detection of molecular aberrancies in CD34+CD38– ALDHbright and ALDHlow compartments of CD34-positive AML. The FLT3-ITD percentage is determined in the total leukemic blast population (data not shown), the CD34– cell population and the CD34+CD38-ALDHlow and CD34+CD38-ALDHbright compartments. The NPM1 mutation analysis is not quantative. # the ALDHbright compartment contained in one case a small mutant peak (AML-945), likely caused by relatively poor separation of ALDHbright and ALDHlow populations due to overlap in the boundary region.