Dendritic Cells

To evaluate how conventional flow cytometry assays can alter dendritic cell enumeration counting in comparison with minimal sample perturbation protocols, in terms of absolute cell counting, percentage and stain index of peripheral blood dendritic cells subsets.
Conclusion:
We have demonstrated that minimal sample perturbation methods may have advantages over widely accepted protocols for DC detection. Our study shows that PBDCs identification and counting can be heavily affected when erythrolytic solutions are used, since these stellate-shaped populations appear to be more labile with sample manipulation. Importantly, cell concentration, frequency, and the SI were significantly altered. Recognizing and avoiding artifacts will result in more accurate results, and methods allowing minimal cell manipulation are recommended, such as those involving rare cell detection. Performance tracking beads (daily)