H3K9me3 and CENP-A ChIP-seq from Mus spretus and Mus musculus

Centromeres and pericentromeres typically form on highly repetitive satellite DNA. However, whether DNA sequence contributes to centromere and pericentromere organization remains unclear. Mus musculus and Mus spretus provide a model system to determine the principles underlying chromatin organization on different satellite sequences. In Mus musculus, centromeres form on minor satellite, and pericentromeres form on the more abundant major satellite DNA. Mus spretus also has both minor and major satellite, but the relative abundance is reversed. To test whether satellite identities impact centromere and pericentromere formation, we analyzed the DNA sequences underlying centromeres (characterized by CENP-A) and percentromeric heterochromatin (characterized by H3K9me3) by ChIP-seq in Mus spretus.