Identification of Radiosensitivity Gene Signatures Induced by Enzalutamide in Prostate cancer cells

This study aimed to identify gene signatures induced by enzalutamide (ENZA) in hormone-sensitive (LNCaP) and hormone-resistant prostate cancer (PCa) cells. LNCaP and C4-2 cells were treated with ENZA alone or in combination with androgen deprivation therapy (ADT) and radiation (XRT). Through gene expression profiling, we identified that ENZA alone or in combination with ADT enhanced the effect the effect of XRT through immune-related pathways in LNCaP cells and metabolic pathways in C4-2 cells.Kaplan-Meier curve and Cox propotional hazard models showed that low expression of all the candidate genes except PTPRN2 were associated with tumor progression and recurrence in a PCa cohort. Total RNA was extracted from LNCaP (hormone-sensitive PCa cells) and C4-2 (hormone-resistant PCa cells) under eight conditions, control, enzalutamide, ADT, enzalutamide+ADT, radiation , enzalutamide+Radiation, ADT+Radiation, enzalutamide+ADT+radiation.There were three biological replicate for each condition.Total number of sample is 48.