RNAseq of MDA-MB-231 siCon and siCD81 cells

We conducted an RNA sequencing analysis comparing MDA-MB-231 siCon and siCD81 cells. Overall design: MDA-MB-231 cells were transfected with non-targeting siRNA (siCon) and siCD81 using Dharmafect in triplicate. After 48 hours, the cells were harvested and total RNA of MDA-MB-231 cells was isolated using Trizol, phase separated by chloroform, and extracted by alcohol. Samples were sent to Northwestern University's Center for Genetic Medicine Sequencing core facility for deep sequencing analysis.