Genome sequencing and assembly for 20 wild isolates. Schizosaccharomyces pombe

This study aims to understand the intraspecific genomic divergence of the fission yeast Schizosaccharomyces pombe by generating genome assemblies from PacBio long-read sequencing data. A total of 20 S. pombe strains were sequenced using PacBio RSII or Sequel platform. Their genomes were assembled using three different long-read assemblers (Canu, Raven, and Flye). A total of 60 assemblies were generated, with three assemblies for each strain. The assemblies were polished using both PacBio sequencing reads and Illumina sequencing reads.