File S1 - Characterisation of AmphiAmR11, an Amphioxus (Branchiostoma floridae) D2-Dopamine-Like G Protein-Coupled Receptor

Supplementary Information Bayliss and Evans containing Figures S1-S6. Figure S1. Effect of biogenic amines (A) and synthetic agonists (B) on forskolin-stimulated cAMP levels in AmphiAmR11-expressing CHO-K1 cells. Figure S2. Effect of yohimbine and WB4101 on tyramine-induced decreases in forskolin-stimulated cAMP levels in AmphiAmR11-expressing CHO-K1 cells. Figure S3. Time courses for the effect of dopamine and tyramine on ERK1/2 phosphorylation in AmphiAmR11-expressing CHO-K1 cells. Figure S4. The relative effectiveness of the biogenic amines and synthetic agonists on ERK1/2 activation in AmphiAmR11-expressing CHO-K1 cells. Figure S5. Calcium mobilisation in AmphiAmR11-expressing and wild type CHO-K1 cells. Figure S6. Localization of AmphiAmR11 in CHO-K1 cells. (DOCX)