pp1a cleaves itself

The crucial step of autocleavage of pp1a involves the formation of an "intermediate" pp1a dimer which has weak protease activity. This "embedded" 3CLp liberates itself by cleaving the ends off its monomer in trans. Only after that occurs does the cleaved 3CLp form a dimer, the most efficient form of the enzyme (Noske et al, 2021).

pp1a 的自切割关键步骤涉及形成一种“中间态”的 pp1a 二聚体，该二聚体具有微弱的蛋白酶活性。此“嵌入”的 3CLp 通过在其单体末端进行切割而释放自身。唯有此过程发生后，切割的 3CLp 方可形成二聚体，这是酶最有效的形式（Noske 等，2021）。