In Vivo Analysis of Cajal Body Movement, Separation, and Joining in Live Human Cells

Figure 4. Analysis of Cajal body antigens in CBs and mini-CBs. Fluorescence micrographs of HeLaGFP-coilin cells immunolabeled with antibodies specific for known Cajal body antigens. Maximum intensity projection images of deconvolved data sets are shown with GFP-coilin in green (A, C, E, and G) and either fibrillarin (B), Sm proteins (D), SMN (F), and SIP1 (H) in red. Fibrillarin and the Sm proteins (B and D) are detected in CBs (magenta arrowheads) but not in all of the mini-CBs (blue arrowheads). SMN and SIP1 (F and H) are detected in both CBs (magenta arrowheads) and mini-CBs (blue arrowheads). Bar, 5 µm.