Adult type Maturation of Human Induced Pluripotent Stem Cells Derived Cardiomyocytes

Cardiac tissue constructs from human induced pluripotent stem cells (hiPSCs) have been heralded for their possibilities to serve as disease models, utilized in drug screening and cardiac regeneration. That potential remains to be realized because human iPSC derived cardiomyocytes (hiPSC-CMs) are immature, and do not reflect the characteristics of adult human myocytes. Here, we present a method to accelerate hiPSC-CM maturation by maintaining them on a substrate, Cardiac Mimetic Matrix (CMM), which mimics the adult human heart matrix ligand chemistry and submicron ultrastructure. Overall design: 3 replicates each of human induced pluripotent stem cells, were differentiated and matured in 3 different conditions: 30 days maturation (d30), 60 days prolonged maturation (d60), and maturation on our Cardiomimetic Matrix (CMM). A total of 9 samples were seqeunced.