Strength of selection potentiates distinct adaptive responses in an evolution experiment with outcrossing yeast

Selection intensity is expected to influence the magnitude and genetic architecture of adaptive responses, yet it is rarely evaluated as a standalone variable in experimental evolution studies. Here, we evolved outcrossing populations of Saccharomyces cerevisiae for ~200 generations across a spectrum of environmental stress from zero to moderate to high ethanol exposure, to examine how genomic responses vary with stress intensity. Across treatments, adaptation proceeded through many subtle allele and haplotype frequency shifts rather than large changes at single loci, consistent with a highly polygenic response. At loci associated with ethanol adaptation, the high stress treatment led to larger allele frequency changes compared to the moderate or no ethanol stress treatments, with the genomic architecture of adaptation becoming increasingly polygenic as selection intensity decreased. Moderate and high stress conditions engaged partially distinct biological pathways, indicating that sele..., Data is from an evolution experiment with outcrossing yeast populations derived from the \"S12\" base population, which originated from 12 haploid founder strains. Sixty replicate populations were established: 20 control (C), 20 moderate ethanol stress (M, 6% ethanol), and 20 high ethanol stress (H, 10% ethanol). Populations underwent weekly outcrossing and batch culture for 15 cycles, corresponding to approximately 200 generations. Growth rate assays were conducted at the end of the experiment to quantify adaptation by measuring optical density over 48 hours in control and treatment-specific media. DNA was extracted from pooled populations at three timepoints (cycles 1, 7, and 15) and sequenced using Illumina PE150. Raw reads were aligned to the S. cerevisiae S288C reference genome, and SNPs were called using GATK. Variant tables were filtered to retain high-confidence polymorphic sites. Candidate SNPs responding to selection were identified using a modified Cochran-Mantel-Haen..., # Data from: Strength of selection potentiates distinct adaptive responses in an evolution experiment with outcrossing yeast

## Authors

Mark A. Phillips, Megan Sandoval-Powers, Rupinderjit K. Briar, Marcus Scaffo, Shenghao Zhou, Molly K. Burke

## Corresponding Authors

**Mark A. Phillips** (corresponding author)
Email: [[e-mail]](mailto:[e-mail])

**Megan Sandoval-Powers** (co-corresponding author)
Email: [[e-mail]](mailto:[e-mail])

**Molly K. Burke** (co-corresponding authors)
Email: [[e-mail]](mailto:[e-mail])

## Description

This dataset contains data associated with the manuscript: Strength of selection potentiates distinct adaptive responses in an evolution experiment with outcrossing yeast

The data are organized into subfolders by analysis category and contain major input and results files used to support the analyses and generate figures presented in the manuscript. These r...,