Gene changes associated with deletion of Esrrg in fast-spiking cortical neurons in mice [1735-192]

Purpose: The goal of this study is to understand what gene changes are associated with removal of Esrrg in parvalbumin-positive neurons from the mouse cortex using BAC-TRAP Methods: Cortex from mice expressing the L10a transgene used for BAC-TRAP method of RNA isolation and PVcre on either WT or ESRRG flox backgrounds, 3-4 monts of age; two hemispheres/mouse were then extracted and flash frozen on dry ice. RNA was isolated using the BAC-TRAP method and samples were sent for sequencing. Cortical fast-spiking interneuron mRNA profiles of wildtype mice compared to mice with Esrrg deletion. All mice were expressing the L10a transgene Pvcre to enable for BAC-TRAP method of RNA-isolation.