Stem-loop RT-qPCR confirmation for miRNAs.

Four differentially expressed miRNAs and four novel miRNAs were selected from the high-throughput sequencing datasets and confirmed by stem-loop RT-qPCR. The results showed a general consistency between the stem-loop RT-qPCR and the high-throughput sequencing. The fold changes (miRNA copy numbers of TGEV-infected ST cell sample vs. miRNA copy numbers of normal ST cell sample) are shown in the diagram. The fold change cutoffs of the upregulated miRNAs and the downregulated miRNAs were 1.5 and 0.67, respectively. “+” and “–” indicate upregulated and downregulated miRNAs, respectively. qRT-PCR Ct threshold: 0.015.Stem-loop RT-qPCR confirmation for miRNAs.