<b>Figure 2. UL12.5 is required for Phase I lytic gene expression during HSV-1 reactivation.</b><b> </b><b>UL12.5 is required for </b><b>Phase I </b><b>lytic gene expression during HSV</b><b>-1</b><b> reactivation</b>

2B. Quantification of the relative viral genome (vDNA - ICP8) in latently infected (0 hours) and reactivated (60 hours) neurons infected with either KOS-SPA and KOS-UL98 measured by qPCR . 2C. Relative expression of lytic mRNAs and LAT in neurons latently infected (0 hours post reactivation) with either KOS-SPA and KOS-UL98 measured by RT-qPCR for ICP27 (IE), UL30 (E), VP16 (L), and gC (L). 2D. Relative expression of lytic mRNAs (ICP27;UL30;VP16;gC) at 18 hours post-reactivation (Phase I). 2E. Relative expression of lytic mRNAs (ICP27;UL30;VP16;gC) at 48 hours post-reactivation (Phase II). 2F. Relative expression of ICP27 mRNA during Phase I reactivation in neurons latently infected with F/L or the rescue virus; F/LR. 2G. Relative expression of ICP27 mRNA during Phase I reactivation in neurons latently infected with AN-1 or the wild-type strain KOS.