RNA-sequencing dataset for Pseudomonas fluorescens PF08 wild-type strain and ΔDR690

This dataset contains the results of an RNA sequencing analysis conducted to investigate the regulatory role of the protein D7M10_RS27690 in biofilm formation in Pseudomonas fluorescens PF08. The study focuses on comparing the transcriptomic profiles of the wild-type strain PF08 and its mutant ΔDR690, which exhibits increased c-di-GMP levels and enhanced biofilm formation due to the deletion of D7M10_RS27690. Specifically, total RNA of P. fluorescens PF08 WT and ΔDR690 was extracted using Trizol, 2 μg RNA was utilized for the stranded RNA sequencing library preparation using Ribo-off rRNA Depletion Kit (Catalog NO. MRZG12324, Illumina) and KCTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08402, Wuhan Seqhealth Co., Ltd. China) following the manufacturer’s instruction. Library products within 200-500 base pairs were enriched and quantified, subsequently were sequenced on the DNBSEQ-T7 sequencer (MGI Tech Co., Ltd., China) utilizing the PE150 model. For each RNA-sequencing sample, three separate libraries were generated. Following the filtration of the raw reads, the clean reads were matched to the genome of P. fluorescens PF08 (GenBank accession number CP032618.1). Genes differentially expressed between groups were identified using the edgeR package (version 3.12.1). |log2 fold change| > 0.32 and an adjusted P value Files Included: QC File: Quality control metrics for RNA-seq data. DEG Analysis: Lists and annotations of differentially expressed genes. GO and KEGG Enrichment Results: Enrichment analysis results detailing the biological pathways and processes affected by the observed DEGs.