Transcriptomic analysis of SW620-conditioned medium-treated THP-1 macrophages

Siglec-5 and Siglec-14, expressed on myeloid cells, share ligand-binding domains but have opposing signaling functions. While these paired receptors regulate macrophage immune responses against bacterial infection, their role in the tumor microenvironment remains unclear. We found that Siglec-14 expression in THP-1 macrophages, when exposed to CRC tumor-conditioned medium, induces a protumoral SPP1+ tumor-associated macrophage phenotype, with enrichment of angiogenic pathways. Overall design: RNA-seq profiling of human monocytic THP-1 cells engineered to express either an empty vector (EV/THP-1), human Siglec-5 (S5/THP-1), or human Siglec-14 (S14/THP-1) were differentiated with PMA and then treated with SW620-conditioned medium for 48 h