Missing Protein Landscape of Human Chromosomes 2 and 14: Progress and Current Status

Within
the C-HPP, the Swiss and French teams are responsible for
the annotation of proteins from chromosomes 2 and 14, respectively.
neXtProt currently reports 1231 entries on chromosome 2 and 624 entries
on chromosome 14; of these, 134 and 93 entries are still not experimentally
validated and are thus considered as “missing proteins”
(PE2–4), respectively. Among these entries, some may never
be validated by conventional MS/MS approaches because of incompatible
biochemical features. Others have already been validated but are still
awaiting annotation. On the basis of information retrieved from the
literature and from three of the main C-HPP resources (Human Protein
Atlas, PeptideAtlas, and neXtProt), a subset of 40 theoretically detectable
missing proteins (25 on chromosome 2 and 15 on chromosome 14) was
defined for upcoming targeted studies in sperm samples. This list
is proposed as a roadmap for the French and Swiss teams in the near
future.