RNA-Seq analysis of 9G8-RNAi intestines in Drosophila

Previous studies from our lab have characterized a group of splicing factors called SR proteins that function to identify intron/exon borders in regulating metabolic homeostasis in the Drosophila fat body. Decreasing the function of one SR protein, 9G8, causes an increase in triglyceride storage; however, the function of 9G8 in other metabolic tissues like the fly intestine is not known. Decreasing 9G8 in the Drosophila intestine results in the accumulation of triglycerides; however, the 9G8 target genes responsible for this phenotype are unknown. To address this question, we performed RNA sequencing on Drosophila intestines with 9G8 levels reduced by RNAi. Drosophila intestine mRNA profiles of yolk-Gal4>9G8-RNAi intestines compared to yolk-Gal4>GFPdsRNA control fat bodies (3 samples of each genotype).