Gene expression analysis of hyper active mutant SPORTS rat [muscle]

To get insight into the genetic characteristics of hyper active mutant line of rat, SPORTS, and the effect of exercise on gene expression, we compared gene expression profiles of exercised SPORTS rat, sedentary SPORTS rat, and sedentary wild type rat. Using RNA extracted from the muscle of these rats, we performed microarray analysis. Subsequent GO analyses revealed that genes belonging to muscle development and glycolysis were upregulated in exercised SPORTS rat compared to sedentary SPORTS rat, and genes related to coagulation were upreguated in sedentary SPORTS rat compared to wild type rat. These results were consistent with phenotypes, such as hyper activity and thrombotic tendency, which were reported for SPORTS rat.为深入探究SPORTS大鼠（SPORTS rat）高活动度突变株的遗传特征以及运动对基因表达的影响，我们对比了运动组SPORTS大鼠、静息组SPORTS大鼠与静息组野生型大鼠的基因表达谱。通过提取上述大鼠肌肉组织中的核糖核酸（RNA），我们开展了基因芯片分析（microarray analysis）。后续的基因本体（Gene Ontology, GO）分析结果显示：相较于静息组SPORTS大鼠，运动组SPORTS大鼠中参与肌肉发育与糖酵解过程的基因表达上调；相较于野生型大鼠，静息组SPORTS大鼠中与凝血相关的基因表达上调。上述结果与此前已报道的SPORTS大鼠的高活动度及血栓形成倾向等表型特征相符。