SSOM-mCherry transfer requires TraA.
收藏Figshare2016-02-24 更新2026-04-29 收录
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A 1∶1 mixture of donor and recipient cells were mixed and spotted on ½ CTT 1% agar and incubated for 4 hrs. Swarms were harvested and single cells were microscopically examined on glass slides to test whether GFP recipients became red by obtaining SSOM-mCherry. White arrows highlight two cells where transfer occurred. Column micrographs (100× objective) were of identical fields. Rod shaped M. xanthus cells were ∼0.5×6.0 microns. Strains were as described in Figure 2 and were traA+ unless indicated otherwise in column headers.
创建时间：
2016-02-24
