Small molecule-inducible RNA-targeting systems for temporal control of RNA regulation. Small molecule-inducible RNA-targeting systems for temporal control of RNA regulation

Programmable RNA-targeting tools provide the unique opportunity to study RNA regulation and control gene expression in an endogenous environment. However, the temporal control over these systems is lacking, rendering studies on the temporal control of regulation challenging. Here, we report the development of a small molecule-controllable RNA effector system to overcome this challenge. Overall design: RNA-seq experiments were performed on mRNA extracted from HEK-293T cells with one biological replicates; we sequenced two conditions: without small molecule (- ABA) and with small molecule (+ABA)