Several angiosperm plants ddRAD-seq raw sequence reads

The goal of this project is to develop an optimized double digest RAD library preparation protocol be accessible to most angiosperm plant species without much startup pre-experiment and costs. To assess the performance of this approach, we got empirical results from the model species Oryza sativa L. spp. japonica and a near-model species Zea mays L. We also explore repeatability by testing the effectiveness of the method in Phyllostachys heterocycla and Alloteropsis semialata (R. Br.) Hitchc. Finally, we were able to reconstruct phylogenetic relationships of two woody bamboos genera, Dendrocalamus and Phyllostachys with this modified ddRAD (MiddRAD) Protocol.