Ribosome profiling of WT vs Cas9-based DBP1 deletion
收藏Alliance of Genome Resources2026-08-01 收录
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Previous deleitons of the DBP1 locus suffered from an off-target effect due to cassette insertion (Powers et al, eLife, 2022). Here, we made a markerless deletion to assess gene expression in meiosis with and without Dbp1. Although it is normally highly expressed in this context, its deletion does not cause a major defect in meiotic progression or gene expression.
