Ribosome profiling of WT vs Cas9-based DBP1 deletion

Previous deleitons of the DBP1 locus suffered from an off-target effect due to cassette insertion (Powers et al, eLife, 2022). Here, we made a markerless deletion to assess gene expression in meiosis with and without Dbp1. Although it is normally highly expressed in this context, its deletion does not cause a major defect in meiotic progression or gene expression.此前针对DBP1位点的基因敲除实验均因插入盒插入（cassette insertion）引发了脱靶效应（off-target effect）（Powers等，eLife，2022）。本研究构建了无标记敲除体系，用以评估存在与缺失Dbp1时的减数分裂基因表达情况。尽管Dbp1在该过程中通常呈高表达状态，但其缺失并不会对减数分裂进程或基因表达造成显著缺陷。