Characterization of CoCas9 nuclease from Capnocytophaga ochracea
收藏Figshare2023-09-25 更新2026-04-28 收录
下载链接：
https://figshare.com/articles/dataset/Characterization_of_CoCas9_nuclease_from_i_Capnocytophaga_ochracea_i_/24189998
下载链接
链接失效反馈官方服务：
资源简介：
Cas9 nucleases are widely used for genome editing and engineering. Cas9 enzymes encoded by CRISPR-Cas defence systems of various prokaryotic organisms possess different properties such as target site preferences, size, and DNA cleavage efficiency. Here, we biochemically characterized CoCas9 from Capnocytophaga ochracea, a bacterium that inhabits the oral cavity of humans and contributes to plaque formation on teeth. CoCas9 recognizes a novel 5’-NRRWC-3’ PAM and efficiently cleaves DNA in vitro. Functional characterization of CoCas9 opens ways for genetic engineering of C. ochracea using its endogenous CRISPR-Cas system. The novel PAM requirement makes CoCas9 potentially useful in genome editing applications.
创建时间：
2023-09-25
