Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression [Cut & Tag]

Anillin (ANLN), a mitotic protein that regulates contractile ring assembly, has been reported as an oncoprotein. However, the function of ANLN in cancer cells, especially in the nucleus, has not been fully understood. Here, we report a previously unknown role of nuclear ANLN in gene transcriptional regulation. We found that nuclear ANLN directly interacts with the RNA polymerase II (Pol II) large subunit. ANLN enhances initiated Pol II clustering and promotes Pol II CTD phase separation. Short-term depletion of ANLN alters the chromatin binding and enhancer-mediated transcriptional activity of Pol II. The target genes of ANLN-Pol II axis are involved in oxidoreductase activity, Wnt signaling and cell differentiation. Our results reveal the function of nuclear ANLN in transcriptional regulation, providing a theoretical basis for ESCC treatment. CUT&Tag analysis of ANLN and Pol II in esophageal squamous cell carcinoma cells (KYSE150 cells). After knockdown of ANLN in KYSE150 cells for 24 hours, Pol II and H3K27ac were analyzed by CUT&Tag.