Expression of mRNA from MCF10A and UFH-001 human cell lines. Expression of mRNA from MCF10A and UFH-001 human cell lines

UFH-001 cells, a newly isolated breast cancer line, have an STR profile that is most similar to that of the control MCF10A cells. Yet, the UFH-001 line is tumor forming with a triple negative phenotype. These cells have a unique transcriptome profile associated numerous breast cancer marker genes. We used microarrays to detail the global programme of gene expression underlying the metastatic behavior of the UFH-001 cells compared to the MCF10A cells. Overall design: UFH-001 cells were isolated using carbonic anhydrase IX expression as a tag in FACS analysis. RNA was isolated from the sorted cells and authenticated MCF10A cells for microarray analysis using the Affymetrix platform to identify genes underlying the aggressive behavior of the UFH-001 cells.