miRNA expression of Pinus pinaster roots under ammonium nutrition

In the present study, the miRNA expression changes in the maritime pine roots from seedlings of one-month old under different levels of ammonium nutrition was analyzed. Overall design: Pine seedlings were subdivided into three different groups. The control group was irrigated with 80 mL of water (C), the second group was supplied with 80 mL of 0.1 mM NH4Cl and the third group was supplied with 80 mL of 3 mM NH4Cl. Root samples were collected before the irrigation and at two different time points after the treatments (2 and 24 hours). Ten seedlings were collected and pooled per each sample. The 21 samples were sequenced on an Illumina HiSeq 4000 as single-end reads of 50 bp length.