Oral administration of green tea Epigallocatechin-3-gallate reduces oxidative stress and enhances restoration of cardiac function in diabetic rats receiving autologous transplantation of adipose-derived stem cells

Cardio-dysfunction is one of the complications in patients with diabetes mellitus (DM). This paper aimed to investigate if oral administration of green tea Epigallocatechin-3-gallate (EGCG, E) and transplantation of adipose-derived stem cells (ADSC) show cross effects on the treatment of cardiomyopathy in rats with type 1 DM. Wistar male rats were divided into four groups (each group contained 8 animals) including sham, DM (diabetic group), DM + ADSC (DM group with ADSC treatment) and DM + ADSC + E (DM + ADSC group with oral administration of EGCG). Pathological parameters including hypertrophy, inflammation, and fibrosis were activated in DM group. By contrast, all parameters were significantly improved in treatment group (DM + ADSC group). In addition, improvement of pathological parameters in DM + ADSC + E was significantly better than DM + ADSC. We found that EGCG can increase expression of survival marker in ADSC under high glucose environment and reduce serum oxidative stress in DM rats.心功能障碍是糖尿病（Diabetes Mellitus, DM）患者的常见并发症之一。本研究旨在探讨口服绿茶来源的表没食子儿茶素没食子酸酯（Epigallocatechin-3-gallate, EGCG，简称E）联合脂肪来源干细胞（adipose-derived stem cells, ADSC）移植，对1型糖尿病大鼠心肌病的治疗是否存在协同效应。实验将Wistar雄性大鼠分为4组（每组8只），分别为假手术组、糖尿病模型组（DM组）、ADSC治疗糖尿病组（DM+ADSC组）以及EGCG口服干预的ADSC治疗糖尿病组（DM+ADSC+E组）。结果显示，糖尿病模型组大鼠的心肌肥厚、炎症反应及纤维化等病理参数均出现异常激活；与之相比，ADSC治疗组（DM+ADSC组）的上述病理指标均得到显著改善。此外，DM+ADSC+E组的病理参数改善效果显著优于DM+ADSC组。本研究进一步发现，在高糖环境下，EGCG可上调ADSC的存活标志物表达水平，并可降低糖尿病大鼠的血清氧化应激水平。