Activation of WNT signaling in CD4+ T cells promotes immune suppression in pancreatic cancer

We utilized the inducible conditional knockout Tcf7 mouse model to specifically deplete Tcf7 in CD4+ T cells by providing mice with tamoxifen, and then orthotopically inject KPC cells into the pancreas to develop tumor. Single cell RNA sequencing revealed that CD4+ T cells are reprogrammed after Tcf7 depletion to shape the immune microenvironment during pancreatic tumorigenesis. This 10X Genomics single cell RNA sequencing repository includes raw and processed data files from orthotopic tumors in Cd4-CreERT2;Tcf7fl/fl or Cd4-CreERT2;Tcf7+l+ mice treated with tamoxifen. Single cell cDNA libraries were prepared and sequenced at the University of Michigan Advanced Genomics Core using the 10x Genomics platform. The sample was run using paired-end 50 cycle reads on NovaSeq 6000 (Illumina) to a depth of 100,000 reads. Alignment was performed by the University of Michigan Advanced Genomics Core. Parameters include Cell Ranger version 4.0.0 (default settings), initial expected cell count of 20,000.