H3K36me3 protects the mouse epigenome from single nucleotide variations [ChIP-Seq]

We analyze high-throughput profiling of histone H3K36me3 in mouse embryonic stem cells from chromatin immunoprecipitated DNA captured by H3K36me3-specific antibody. We find that H3K36me3 distribution is like expected and enriched at transcribing gene bodies especially with enrichment at 3’-terminal of gene body, which is identical with findings observed in other mammalian cells.本研究针对小鼠胚胎干细胞中的组蛋白H3K36me3开展高通量谱型分析，所用实验材料为经H3K36me3特异性抗体捕获的染色质免疫沉淀DNA。研究发现，H3K36me3的分布符合预期，在转录中的基因体区域存在富集，尤其集中于基因体的3'端区域，该结果与其他哺乳动物细胞中的已有研究发现完全一致。

搜集汇总

背景与挑战

背景概述

该数据集通过ChIP-Seq技术分析小鼠胚胎干细胞中组蛋白修饰H3K36me3的分布，发现其富集在转录基因体，特别是3'末端，这与其他哺乳动物细胞的研究一致。数据集包含4个样本，总大小为20.27 GB，聚焦于H3K36me3在保护表观基因组免受单核苷酸变异影响的作用。