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Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors [iR_iHEC]

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NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE129444
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We inserted the inducible expression cassette of Runx1 into the Rosa26 locus of embryonic stem cells. Based on a modified protocol for HEC induction from PSC, we successfully generated iHEC Under the haematopoietic induction process (iR-HEC). mRNA profiles of single hematopoietic endothelia cells (iR-iHEC). single iR-iHECs were sorted from PSC-derived cells for RNAseq analysis according to kit instruction of Discover-sc WTA Kit V2(Vazyme) and TruePrepTM  DNA Library Prep Kit V2 (Vazyme), using illumina HiSeq X Ten.
创建时间:
2020-11-07
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