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Fusion of different epitope tags to N-terminus of BST-2 ΔN20 results in different effects on antiviral function.

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Figshare2016-02-23 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Fusion_of_different_epitope_tags_to_N_terminus_of_BST_2_916_N20_results_in_different_effects_on_antiviral_function_/1219004
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(A and B) BST-2 ΔN20 IHA, BST-2 ΔN20 NHA, BST-2 ΔN20 NT, BST-2 ΔN20 NMyc, BST-2 ΔN20 NFlag, BST-2 ΔN20 NHARD or control vector VR1012 (25 ng each) was co-transfected with 1 µg of the proviral plasmid pNL4-3 ΔVpu or pNL4-3 WT in 293T cells. At 48 h post-transfection, cultured supernatants were ultracentrifuged to concentrate the virus particles. Virions and cell lysates were analyzed by Western blotting. This experiment was repeated for three times and the most representative data was shown.
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2016-02-23
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