QTL-seq for identification of loci associated with resistance to soybean red crown rot

To identify QTLs for resistance to soybean red crown rot caused by the fungus Calonectria ilicicola, QTL-seq analyses were performed using resistant (R) bulk and susceptible (S) bulk of two populations of recombinant inbred lines derived from crosses between one of two wild soybean accessions, Gs-7 and Gs-9 (resistant), to a soybean cultivar, Enrei (susceptible). One population, En7 (EnreiÃ?Gs-7), consisted of 185 lines; and another population, En9 (EnreiÃ?Gs-7) consisted of 249 lines. Each 9-10 lines from the most resistant lines and the most susceptible lines were selected to construct R and S bulks, respectively.