Immunofluorescence histochemical staining of the total nerve fiber and sympathetic nerve fiber in deep infiltrating endometriotic specimens of uterosacral ligament and healthy uterosacral ligament.

Total nerve fibers were orange-yellow stained by Anti-PGP 9.5 antibody (A: EAN-USL-EM; B: PEN-USL-EM; C: N-USL-C). Sympathetic nerve fibers were also orange-yellow stained by Anti-TH antibody (D: EASN-USL-EM; E: PESN-USL-EM; F: SN-USL-C). Ectopic endometrial glandular epithelial cells was stained in green by anti-Sema 3A antibody staining (A was the merge image of double staining of both Sema 3A and PGP 9.5, D was the merge image of double staining of both Sema 3A and TH), nuclei were stained in blue by DAPI staining. White triangle: orange-yellow stained nerve fibers. (Original magnification 200×)